Instructions for use:

1. Add Colikat Rapid
Presence/ Absence: Add 1 bag of COLIKAT RAPID® to 100 ml of water sample. Close the sample vessle and shake until the reagent dissolves.
1.2. Precense Absence Incubate 18h
For presence/absence testing incubate the closed vessel for 18-22 hours at 36°C ± 2°C and read-off the result. The vessels should have room temperature before incubation. If turned yellow coliforms are present. If it shows fluorescence unter UV-light Escherichia coli is present.
2. Colikat Enumeration Tray
Quantification: For quantitative testing pour the 100ml sample into the COLIKAT Enumeration Tray. COLIKAT RAPID® turned yellow coliforms are present. If it shows fluorescence unter UV-light Escherichia coli is present.
3. Colikat Seal Applicator
Seal the COLIKAT Enumeration Tray with the COLIKAT Seal applicator or another enumeration tray sealing device.
4. Incubate 18h 2
Incubate the sample 18-22 hours at 36°C ± 2°C. Count the yellow wells of COLIKAT Enumeration Tray (= coliforms). Count the yellow wells of the COLIKAT Enumeration Tray that show fluorescence under an UV light (365nm) in a dark environment (= Escherichia coli).
5. Mpn Form
Transform the count results to the number of CFU the sample by using the MPN table in the appendix to EN ISO 9308-2.

Additional notes:

  • The color of the sample can change slightly after addition of the COLIKAT RAPID® reagents. 
  • Please note that stationary regulation might defer from the procedure described in these instructions of use.
  • COLIKAT RAPID® normally does not show foam forming. In the unlikely event of excess foam forming usa an Antifoam Agent. 
  • COLIKAT RAPID® can be used to determine the MPN using a standard MPN-multiple tube format.
  • Quality control for COLIKAT RAPID® is carried out according to EN ISO 11133 an EN ISO 9308-2. 
  • Always follow aseptic working techniques. Dispose according to local rules and legislation.
Download the instructions for use

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40597 Düsseldorf

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