Instructions for use:
Presence/ Absence: Add 1 bag of COLIKAT RAPID® to 100 ml of water sample. Close the sample vessle and shake until the reagent dissolves.
For presence/absence testing incubate the closed vessel for 18-22 hours at 36°C ± 2°C and read-off the result. The vessels should have room temperature before incubation. If turned yellow coliforms are present. If it shows fluorescence unter UV-light Escherichia coli is present.
Quantification: For quantitative testing pour the 100ml sample into the COLIKAT Enumeration Tray.
Seal the Enumeration Tray with the XDG Multiple Well MPN Tray Sealer or another enumeration tray sealing device.
Incubate the sample 18-22 hours at 36°C ± 2°C. Count the yellow wells of COLIKAT Enumeration Tray (= coliforms). Count the yellow wells of the COLIKAT Enumeration Tray that show fluorescence under an UV light (365nm) in a dark environment (= Escherichia coli).
Transform the count results to the number of CFU the sample by using the MPN table in the appendix to EN ISO 9308-2.
Additional notes:
- Shelf life: 24 month. Shelf life after initial opening: 6 month.
- The color of the sample can change slightly after addition of the COLIKAT RAPID® reagents.
- Please note that stationary regulation might defer from the procedure described in these instructions of use.
- COLIKAT RAPID® normally does not show foam forming. In the unlikely event of excess foam forming usa an Antifoam Agent.
- COLIKAT RAPID® can be used to determine the MPN using a standard MPN-multiple tube format.
- Quality control for COLIKAT RAPID® is carried out according to EN ISO 11133 an EN ISO 9308-2.
- Always follow aseptic working techniques. Dispose according to local rules and legislation.